быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
POU2F1/OCT1 Rabbit mAb (A3683)
- Описание
- Характеристики
-
Overview
Product name POU2F1/OCT1 Rabbit mAb Catalog No. A3683 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0822 Background
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human POU2F1/POU2F1/OCT1 (P14859). Sequence MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMT Gene ID Swiss Prot Synonyms OCT1; OTF1; Oct1Z; oct-1B Calculated MW 76kDa Observed MW 90kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, SGC-7901, Mouse testis Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using POU2F1/POU2F1/OCT1 Rabbit mAb (A3683) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using POU2F1/POU2F1/OCT1 Rabbit mAb (A3683) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using POU2F1/POU2F1/OCT1 Rabbit mAb (A3683) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human POU2F1/POU2F1/OCT1 (P14859). Isotype IgG