быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
FKBP38/FKBP8 Rabbit mAb (A8701)
- Описание
- Характеристики
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Overview
Product name FKBP38/FKBP8 Rabbit mAb Catalog No. A8701 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1259 Background
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Unlike the other members of the family, this encoded protein does not seem to have PPIase/rotamase activity. It may have a role in neurons associated with memory function.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FKBP38/FKBP8 (Q14318). Sequence MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNG Gene ID Swiss Prot Synonyms FKBP38; FKBPr38 Calculated MW 45kDa Observed MW 49kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HepG2, Jurkat, Mouse brain, Mouse kidney, Rat brain Cellular location Cytoplasmic side, Mitochondrion membrane, Single-pass membrane protein 
Western blot analysis of extracts of various cell lines, using FKBP38/FKBP8 Rabbit mAb (A8701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using FKBP38/FKBP8 Rabbit mAb (A8701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of C6 cells using FKBP38/FKBP8 Rabbit mAb (A8701) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using FKBP38/FKBP8 Rabbit mAb (A8701) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FKBP38/FKBP8 (Q14318). Isotype IgG