быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ATGL/PNPLA2 Rabbit mAb (A5126)
- Описание
- Характеристики
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Overview
Product name ATGL/PNPLA2 Rabbit mAb Catalog No. A5126 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1192 Background
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 300-394 of human ATGL / PNPLA2 (NP_065109.1). Sequence LPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLP Gene ID Swiss Prot Synonyms ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik Calculated MW 55kDa Observed MW 55kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, A-431, U-87MG, Mouse brain, Rat brain, Mouse kidney, Rat kidney, Mouse heart, Rat heart Cellular location Cell membrane, Lipid droplet, Single-pass type II membrane protein Customer validation (Jurkat、RPMI-8226、PC-3、HepG2、HeLa、A549、T47D)
WB(Mus musculus, Homo sapiens )
IHC(Mus musculus)
Western blot analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody (A5126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 300-394 of human ATGL / PNPLA2 (NP_065109.1). Isotype IgG