быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
KDM5A Rabbit mAb (A4755)
- Описание
- Характеристики
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Overview
Product name KDM5A Rabbit mAb Catalog No. A4755 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1120 Background
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1690 of human KDM5A (P29375). Sequence HAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS Gene ID Swiss Prot Synonyms RBP2; RBBP2; RBBP-2 Calculated MW 192kDa Observed MW 200kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP ChIP Recommended dilution - WB 1:500 - 1:2000
- IP 1:100 - 1:500
- ChIP 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa, HepG2, Mouse lung Cellular location Nucleus, nucleolus 
Western blot analysis of extracts of various cell lines, using KDM5A Rabbit mAb (A4755) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Western blot analysis of extracts of Mouse lung, using KDM5A Rabbit mAb (A4755) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KDM5A antibody (A4755). Western blot was performed from the immunoprecipitate using KDM5A antibody (A4755) at a dilution of 1:500.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody (A4755) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1690 of human KDM5A (P29375). Isotype IgG