быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Delta-sarcoglycan (SGCD) Rabbit mAb (A9175)
- Описание
- Характеристики
-
Overview
Product name Delta-sarcoglycan (SGCD) Rabbit mAb Catalog No. A9175 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1463 Background
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 190-289 of human Delta-sarcoglycan (SGCD) (Q92629). Sequence LRLESPTRSLVMEAPKGVEINAEAGNMEATCRTELRLESKDGEIKLDAAKIRLPRLPHGSYTPTGTRQKVFEICVCANGRLFLSQAGAGSTCQINTSVCL Gene ID Swiss Prot Synonyms SGD; DAGD; 35DAG; CMD1L; SGCDP; LGMDR6; SG-delta Calculated MW 32kDa Observed MW 36kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, RD, Mouse heart Cellular location Cell membrane, Cytoplasm, Single-pass type II membrane protein, cytoskeleton, sarcolemma 
Western blot analysis of extracts of various cell lines, using Delta-sarcoglycan (SGCD) Rabbit mAb (A9175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of Mouse heart, using Delta-sarcoglycan (SGCD) Rabbit mAb (A9175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic peptide corresponding to a sequence within amino acids 190-289 of human Delta-sarcoglycan (SGCD) (Q92629). Isotype IgG