быстрая доставка ~4 недели
Размер
- 10 μg
- 20 μg
- 5 μg
Active Recombinant Human FGFR-2/KGFR/CD332 Protein (RP00046)
- Описание
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Overview
Product name Active Recombinant Human FGFR-2/KGFR/CD332 Protein Catalog No. RP00046 Description Active Recombinant Human FGFR-2/KGFR/CD332 Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Pro458-Gln778) of human FGFR-2 (Accession #NP_000132.3). Bio-Activity 1.Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.21-0.84 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGFR2 at 2 μg/mL (100 μL/well) can bind Recombinant Human FGF1 with a linear range of 0.128-48.3 ng/mL. Purity > 97% by SDS-PAGE. Endotoxin < 1.0 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.Contact us for customized product form or formulation. Species Human Background
The protein is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.Product information
Synonym BBDS; BEK; BFR-1; CD332; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25 Expression Host Baculovirus-Infected Sf9 Cells Tag No tag Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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