быстрая доставка ~4 недели
Размер
- 100 μg
- 1000 μg
- 500 μg
Rabbit anti-Rat IFN-gamma mAb (DET) (RM17901)
- Описание
- Характеристики
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Overview
Product name Rabbit anti-Rat IFN-gamma mAb (DET) Catalog No. RM17901 Catagory Elisa Antibody Kit Application ELISA Description Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL.Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. Immunogen Recombinant Rat IFN-gamma protein Purirty Affinity purification Ig Type Rabbit IgG Formulation Supplied as a 0.2um filtered solution in PBS with 0.05%ProClin 300, PH 7.8. CrossReactivity No cross-reactivity in ELISA assay withrecombinant
Rat IL-1 β, Rat IL-2, Rat IL-4, Rat IL-6, Rat IL-10, Rat TNF-α, Human IFN-γ, Mouse IFN-γStorage This antibody can be stored at 2℃-8℃ for one monthwithout detectable loss of activity. Antibody products are stable for twelve months fromdate of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Application
Recommended
ConcentrationSample ELISA Capture 0.5-4ug/mL Rabbit anti-Rat IFN-gamma (CAP)(Cat. No.RM17900 ELISA Detection 0.333-0.0833ug/mL Rabbit anti-Rat IFN-gamma (DET)(Cat. No.RM17901) Standard 15.6-1000pg/mL Recombinant Rat IFN-gamma Protein Rabbit anti-Rat IFN-gamma mAb (DET) images
This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.No significant cross-reactivity or interference was observed.Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
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Antibody type Matched antibody pairs Immunogen Recombinant Rat IFN-gamma protein
