быстрая доставка ~4 недели
Размер
- 100 μg
- 1000 μg
- 500 μg
Rabbit anti-Mouse IL-12/IL-23 p40 mAb (CAP) (RM17947)
- Описание
- Характеристики
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Overview
Product name Rabbit anti-Mouse IL-12/IL-23 p40 mAb (CAP) Catalog No. RM17947 Catagory Elisa Antibody Kit Application ELISA Description IL-12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine produced primarily by antigen-presenting cells (monocytes/macrophages, dendritic cells and B lymphocytes). IL-12 has multiple effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production and Th1 subset development.IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a unique 35 kDa (p35) subunit and a common 40 kDa (p40) subunit that is also present in IL-23. Monomers of the p40 and p35 subunits by themselves do not have IL-12 activity, but the homodimer of p40 has been shown to bind the IL-12 receptor and is an IL-12 antagonist .The genes for mouse p40 and p35, found on chromosomes 11 and 3, respectively, are independently regulated . The expression of p35 mRNA has been found to be nearly ubiquitous. However, p35 subunits that are not dimerized with the p40 subunits are not secreted and have not been detected in culture supernates of cells expressing only p35 or both p35 and p40 mRNAs. In cells expressing both p35 and p40 mRNAs, p40 mRNA is expressed to a higher level and free p40 subunits not associated with p35 subunits are secreted together with heterodimeric IL-12 p70. Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist as monomers. In the culture supernates of various activated human monocytes where free p40 is present in vast excess over p70, the levels of p70 measured by bioassays are consistent with those measured using a p70-specific immunoassay, suggesting that p40 monomers are not efficient IL-12 antagonists. In the mouse system, p40 homodimers are produced in vivo and function as IL-12 antagonists.Polymorphisms exist in the mouse IL-12/IL-23 p40 sequence. The monoclonal capture antibody used in this ELISA kit is allele-specific. It recognizes the IL-12/IL-23 p40 variant with methionine at residue 169 in mouse strains A.SW, BALB/c, C3H, DBA/1, C57BL/6, NOR, and 129/J. The capture antibody does not recognize the M169T IL-12/IL-23 p40 variant present in the AKR, DBA/2, NOD, NZB, NZC, NZO, NZW, and SJL strains . Immunogen Recombinant Mouse IL-12/IL-23 p40 Purirty Affinity purification Ig Type Rabbit IgG Formulation Supplied as a 0.2um filtered solution in PBS with 0.05%ProClin 300, PH 7.8. CrossReactivity No cross-reactivity in ELISA assay withrecombinant
Mouse. G-CSF，Mouse.GM-CSF，Mouse. IL-1a，Mouse.IL-1β，Mouse. IL-4，Mouse.IL-5，Mouse.IL-6， Mouse IL-8， Mouse IL-9，Mouse.IL-10，Mouse.IL-12p70，Mouse.TNF-α，Mouse.Leptin，Mouse.CXCL1/KC；Mouse.IL-13, Human IL-12/IL-23 p40, Human IL-12 p70Storage This antibody can be stored at 2℃-8℃ for one monthwithout detectable loss of activity. Antibody products are stable for twelve months fromdate of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Application
Recommended
ConcentrationSample ELISA Capture 1-4ug/mL Rabbit anti-Mouse IL-12/IL-23 p40 mAb(CAP)(Cat. No.RM17947) ELISA Detection 0.033-0.133ug/mL Rabbit anti-Mouse IL-12/IL-23 p40 mAb(DET)(Cat. No.RM17948) Standard 31.25-2000pg/mL Recombinant Mouse IL-10 Protein Rabbit anti-Mouse IL-12/IL-23 p40 mAb (CAP) images
This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.No significant cross-reactivity or interference was observed.Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
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Antibody type Matched antibody pairs Immunogen Recombinant Mouse IL-12/IL-23 p40
