быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Acetyl-Histone H3-K4 Rabbit mAb (A24340)
- Описание
- Характеристики
-
Overview
Product name Acetyl-Histone H3-K4 Rabbit mAb Catalog No. A24340 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62521 Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic acetylated peptide around K4 of human Histone H3 (NP_003520.1). Sequence ARTK(AC)QTARKSTGGK-C Gene ID Swiss Prot Synonyms H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A Calculated MW 16kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP ChIP DB Recommended dilution - DB 1:2000 - 1:5000
- WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- ChIP 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa TSA, NIH/3T3 TSA, C6 TSA Cellular location Chromosome, nucleus 
Western blot analysis of HeLa, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of NIH/3T3, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of various lysates, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:3000 dilution.
Immunofluorescence analysis of C6 TSA and C6 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa TSA and HeLa cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 TSA and NIH-3T3 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of HeLa TSA cells using 5ug Acetyl-Histone H3-K4 Rabbit mAb (A24340). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody (A24340) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
-
Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic acetylated peptide around K4 of human Histone H3 (NP_003520.1). Isotype IgG