быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Acetyl-Histone H3-K18 Rabbit pAb (A7257)
- Описание
- Характеристики
-
Overview
Product name Acetyl-Histone H3-K18 Rabbit pAb Catalog No. A7257 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic acetylated peptide around K18 of human H3 (NP_003520.1). Sequence PRKQL Gene ID Swiss Prot Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J Calculated MW 15kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:200 - 1:500
- IF/ICC 1:500 - 1:1000
- IP 1:200 - 1:500
- ChIP 1:50 - 1:100
- ChIP-seq 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa Cellular location Chromosome, Nucleus Customer validation WB(Mus musculus, Homo sapiens, Saccharomyces cerevisiae, Monascus ruber, Oryza sativa Japonica Group, Eurosta solidaginis)
CHIP(Homo sapiens)
Western blot analysis of extracts of HeLa cells, using Acetyl-Histone H3-K18 antibody (A7257) at 1:500 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (A7257) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded rat brain using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human gastric cancer using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (A7257) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 antibody (A7257) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (A7257) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb antibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb yantibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
-
Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic acetylated peptide around K18 of human H3 (NP_003520.1). Isotype IgG