![[KO Validated] Emerin/EMD Rabbit pAb (A18030)](/upload/resize_cache/iblock/bc3/340_340_140cd750bba9870f18aada2478b24840a/czj6u3au5vy1qcaphx9qsen90gi1mb7a.jpg "[KO Validated] Emerin/EMD Rabbit pAb (A18030)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Emerin/EMD Rabbit pAb (A18030)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Emerin/EMD Rabbit pAb Catalog No. A18030 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Emerin/Emerin/EMD (NP_000108.1). Sequence MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQD Gene ID Swiss Prot Synonyms STA; EDMD; LEMD5 Calculated MW 29kDa Observed MW 30kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Nucleoplasmic side, Nucleus inner membrane, Nucleus outer membrane, Single-pass membrane protein 
Western blot analysis of extracts from normal (control) and Emerin/Emerin/EMD knockout (KO) 293T cells, using Emerin/Emerin/EMD antibody (A18030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Emerin/Emerin/EMD (NP_000108.1). KO Validated Validated Isotype IgG