![[KO Validated] Carbonic Anhydrase 2 (CA2) Rabbit pAb (A18034)](/upload/resize_cache/iblock/8c5/340_340_140cd750bba9870f18aada2478b24840a/2fkql6f0w52i62bnrk2ydedt5l0yrf50.jpg "[KO Validated] Carbonic Anhydrase 2 (CA2) Rabbit pAb (A18034)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Carbonic Anhydrase 2 (CA2) Rabbit pAb (A18034)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Carbonic Anhydrase 2 (CA2) Rabbit pAb Catalog No. A18034 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Carbonic Anhydrase 2 (CA2) (NP_000058.1). Sequence MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPL Gene ID Swiss Prot Synonyms CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282 Calculated MW 29kDa Observed MW 28kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cell membrane, Cytoplasm 
Western blot analysis of extracts from normal (control) and Carbonic Anhydrase 2 (CA2) knockout (KO) 293T cells, using Carbonic Anhydrase 2 (CA2) antibody (A18034) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Carbonic Anhydrase 2 (CA2) (NP_000058.1). KO Validated Validated Isotype IgG