![[KO Validated] NLRX1 Rabbit pAb (A18065)](/upload/resize_cache/iblock/208/340_340_140cd750bba9870f18aada2478b24840a/ip0reyaopx8pepfl8rm0z84fin3z1k02.jpg "[KO Validated] NLRX1 Rabbit pAb (A18065)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NLRX1 Rabbit pAb (A18065)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NLRX1 Rabbit pAb Catalog No. A18065 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_001269073.1). Sequence AEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPPAELALEHQPPQAGLPPLALSQLFNPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGPAPASLCQLVAQRYTPLKEVLPLMAAAGSHLLFVLHGLEHLNLDFRLAGTGLCSDPEEPQEPAAIIVNLLRKYMLPQASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPYCGYAVGGSGVSATPAQRDHLVQMLSRNLEG Gene ID Swiss Prot Synonyms NOD5; NOD9; NOD26; DLNB26; CLR11.3 Calculated MW 108kDa Observed MW 108kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Mitochondrion outer membrane 
Western blot analysis of extracts from normal (control) and NLRX1 knockout (KO) HeLa cells, using NLRX1 antibody (A18065) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_001269073.1). KO Validated Validated Isotype IgG