![[KO Validated] UBE2C Rabbit pAb (A18075)](/upload/resize_cache/iblock/49f/340_340_140cd750bba9870f18aada2478b24840a/yc5b597d3fx84x07nypyd3x6yvgkz3p1.jpg "[KO Validated] UBE2C Rabbit pAb (A18075)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] UBE2C Rabbit pAb (A18075)
- Описание
- Характеристики
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Overview
Product name [KO Validated] UBE2C Rabbit pAb Catalog No. A18075 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1). Sequence MASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP Gene ID Swiss Prot Synonyms UBCH10; dJ447F3.2 Calculated MW 20kDa Observed MW 18kDa Applications
Reactivity Human Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
- IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa Cellular location cytosol, nucleoplasm, nucleus, plasma membrane Customer validation WB(Homo sapiens)
Western blot analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UBE2C antibody (A18075) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1). KO Validated Validated Isotype IgG