![[KO Validated] TMED10 Rabbit pAb (A18090)](/upload/resize_cache/iblock/1ec/340_340_140cd750bba9870f18aada2478b24840a/wtnnw9gcsq4gddd2f0uxx9yr4lhd8ct8.jpg "[KO Validated] TMED10 Rabbit pAb (A18090)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TMED10 Rabbit pAb (A18090)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TMED10 Rabbit pAb Catalog No. A18090 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-190 of human TMED10 (NP_006818.3). Sequence HLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFS Gene ID Swiss Prot Synonyms p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA) Calculated MW 25kDa Observed MW 21kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus, Melanosome, Single-pass type I membrane protein, cis-Golgi network membrane, secretory vesicle membrane, trans-Golgi Customer validation WB(Homo sapiens)
Western blot analysis of extracts from normal (control) and TMED10 knockout (KO) 293T cells, using TMED10 antibody (A18090) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-190 of human TMED10 (NP_006818.3). KO Validated Validated Isotype IgG