![[KO Validated] PAK2 Rabbit pAb (A18093)](/upload/resize_cache/iblock/51c/340_340_140cd750bba9870f18aada2478b24840a/vxavx5flww94t6ts93w5wep8vq2fu9to.jpg "[KO Validated] PAK2 Rabbit pAb (A18093)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PAK2 Rabbit pAb (A18093)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] PAK2 Rabbit pAb Catalog No. A18093 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PAK2 (NP_002568.2). Sequence MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVD Gene ID Swiss Prot Synonyms KNO2; PAK65; PAKgamma Calculated MW 58kDa Observed MW 58kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Cytoplasm, Cytoplasm, Lipid-anchor, Membrane, Nucleus, perinuclear region 
Western blot analysis of extracts from normal (control) and PAK2 knockout (KO) HeLa cells, using PAK2 antibody (A18093) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PAK2 (NP_002568.2). KO Validated Validated Isotype IgG