![[KO Validated] Cyclin B1 Rabbit mAb (A19037)](/upload/resize_cache/iblock/b40/340_340_140cd750bba9870f18aada2478b24840a/kq5fh7x04tbw53b6th8se9gx46ge0htx.jpg "[KO Validated] Cyclin B1 Rabbit mAb (A19037)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Cyclin B1 Rabbit mAb (A19037)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Cyclin B1 Rabbit mAb Catalog No. A19037 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0474 Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635). Sequence LRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAY Gene ID Swiss Prot Synonyms CCNB Calculated MW 48kDa Observed MW 55kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat, Mouse testis, HeLa Cellular location Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center Customer validation IHC(Homo sapiens,Mus musculus)
IF(Homo sapiens,Mus musculus)
WB(Homo sapiens, Mus musculus)
Western blot analysis of extracts from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells, using Cyclin B1 antibody (A19037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (A19037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635). KO Validated Validated Isotype IgG