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[KO Validated] Cyclin D1 Rabbit mAb (A19038)

быстрая доставка ~4 недели

Размер

100 μL
200 μL
50 μL

Описание
Характеристики

Overview

Product name	[KO Validated] Cyclin D1 Rabbit mAb
Catalog No.	A19038
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0300

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).
Sequence	ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Gene ID
Swiss Prot
Synonyms	BCL1; PRAD1; U21B31; D11S287E
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2 , MCF7 , HeLa , Mouse lung
Cellular location	Nucleus
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Mus musculus) Western Blot(Mus musculus) Co-IP(Homo sapiens) WB(Homo sapiens) IF(Mus musculus, Rattus norvegicus) WB，IHC(Homo sapiens) RT-qPCR(Homo sapiens)

Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse colon using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (A19038, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Key applications	Western blotting, Immunohistochemistry, Immunofluorescence
Host species	Кролик
Antibody type	Primary Antibodies
Reactivity	Человек, Мышь, Крыса
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).
KO Validated	Validated
Isotype	IgG