![[KO Validated] ATG5 Rabbit mAb (A19677)](/upload/resize_cache/iblock/b59/340_340_140cd750bba9870f18aada2478b24840a/ilf2mnoq67ov14jy4ggt4aotd8efia83.jpg "[KO Validated] ATG5 Rabbit mAb (A19677)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] ATG5 Rabbit mAb (A19677)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] ATG5 Rabbit mAb Catalog No. A19677 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0156 Background
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0). Sequence MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNI Gene ID Swiss Prot Synonyms ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE Calculated MW 32kDa Observed MW 55kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples 293T, HeLa, SH-SY5Y, Mouse brain, Mouse lung Cellular location Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane Customer validation (293T, Hela,A549,HepG2,PC-3,HL-60,RPMI-8226,MLOT-4 )
WB(Spodoptera frugiperda, Mus musculus, Homo sapiens, Human, Rattus norvegicus)
Western Blot(Mus musculus)
Western blot analysis of extracts from wild type (WT) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human placenta using ATG5 antibody (A19677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KO Validated] ATG5 Rabbit mAb (A19677). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:1000.
-
Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0). KO Validated Validated Isotype IgG