![[KO Validated] NRD1 Rabbit pAb (A19857)](/upload/resize_cache/iblock/b25/340_340_140cd750bba9870f18aada2478b24840a/p5c57b9617ha5rkfib6r2k0ig2jxelse.jpg "[KO Validated] NRD1 Rabbit pAb (A19857)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NRD1 Rabbit pAb (A19857)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NRD1 Rabbit pAb Catalog No. A19857 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a zinc-dependent endopeptidase that cleaves peptide substrates at the N-terminus of arginine residues in dibasic moieties and is a member of the peptidase M16 family. This protein interacts with heparin-binding EGF-like growth factor and plays a role in cell migration and proliferation. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human NRD1 (NP_001095132.1). Sequence SGAEIEDDDEEGFDDEDEFDDEHDDDLDTEDNELEELEERAEARKKTTEKQSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPRKNNIDTHARLREFWMRYYSSHYMTLVVQSKETLDTLEKWVTEIF Gene ID Swiss Prot Synonyms NRD1; hNRD1; hNRD2 Calculated MW 132kDa Observed MW 131kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location cell surface, cytosol, mitochondrion 
Western blot analysis of extracts from normal (control) and NRD1 knockout (KO) 293T cells, using NRD1 antibody (A19857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human NRD1 (NP_001095132.1). KO Validated Validated Isotype IgG