![[KO Validated] SPR Rabbit pAb (A19859)](/upload/resize_cache/iblock/327/340_340_140cd750bba9870f18aada2478b24840a/2ail44c7wnu1j2c1tgk61nmjbzehfy0e.jpg "[KO Validated] SPR Rabbit pAb (A19859)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SPR Rabbit pAb (A19859)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SPR Rabbit pAb Catalog No. A19859 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human SPR (NP_003115.1). Sequence MEGGLGRAVCLLTGASRGFGRTLAPLLASLLSPGSVLVLSARNDEALRQLEAELGAERSGLRVVRVPADLGAEAGLQQLLGALRELPRPKGLQRLLLINNAGSLGDVSKGFVDLSDSTQVNNYWALNLTSMLCLTSSVLKAFPDSPGLNRTVVNISSLCALQPFKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYDK Gene ID Swiss Prot Synonyms SDR38C1 Calculated MW 28kDa Observed MW 28kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cytoplasm 
Western blot analysis of extracts from normal (control) and SPR knockout (KO) HeLa cells, using SPR antibody (A19859) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human SPR (NP_003115.1). KO Validated Validated Isotype IgG