![[KO Validated] ANXA3 Rabbit pAb (A19861)](/upload/resize_cache/iblock/636/340_340_140cd750bba9870f18aada2478b24840a/i1rl9zpnicws79ezavrxfo3kkgdxoh8x.jpg "[KO Validated] ANXA3 Rabbit pAb (A19861)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] ANXA3 Rabbit pAb (A19861)
- Описание
- Характеристики
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Overview
Product name [KO Validated] ANXA3 Rabbit pAb Catalog No. A19861 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1, 2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ANXA3 (NP_005130.1). Sequence MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD Gene ID Swiss Prot Synonyms ANX3 Calculated MW 36kDa Observed MW 34kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location axon, cytoplasm, dendrite, extracellular exosome, plasma membrane 
Western blot analysis of extracts from normal (control) and ANXA3 knockout (KO) HeLa cells, using ANXA3 antibody (A19861) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ANXA3 (NP_005130.1). KO Validated Validated Isotype IgG