![[KO Validated] TRIM25 Rabbit pAb (A19887)](/upload/resize_cache/iblock/34a/340_340_140cd750bba9870f18aada2478b24840a/3dq6qlp13a7o0z115gvhsqc8tytrh25k.jpg "[KO Validated] TRIM25 Rabbit pAb (A19887)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TRIM25 Rabbit pAb (A19887)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TRIM25 Rabbit pAb Catalog No. A19887 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2). Sequence SPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALP Gene ID Swiss Prot Synonyms EFP; Z147; RNF147; ZNF147 Calculated MW 71kDa Observed MW 71kDa Applications
Reactivity Human, Mouse Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples A-549 Cellular location Cytoplasm Customer validation WB(Homo sapiens)
Western blot analysis of extracts from normal (control) and TRIM25 knockout (KO) A-549 cells, using TRIM25 antibody (A19887) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10S. -
Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2). KO Validated Validated Isotype IgG