![[KO Validated] NUP210 Rabbit pAb (A19895)](/upload/resize_cache/iblock/a07/340_340_140cd750bba9870f18aada2478b24840a/l7gudl9wilgqhz59555f3q4na2gqcap3.jpg "[KO Validated] NUP210 Rabbit pAb (A19895)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NUP210 Rabbit pAb (A19895)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NUP210 Rabbit pAb Catalog No. A19895 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 680-880 of human NUP210 (NP_079199.2). Sequence QNVTAEDTDSIGLALFAPHSSRNYQQHWILVTCQALGEQVIALSVGNKPSLTNPFPAVEPAVVKFVCAPPSRLTLAPVYTSPQLDMSCPLLQQNKQVVPVSSHRNPRLDLAAYDQEGRRFDNFSSLSIQWESTRPVLASIEPELPMQLVSQDDESGQKKLHGLQAILVHEASGTTAITATATGYQESHLSSARTKQPHDPL Gene ID Swiss Prot Synonyms GP210; POM210 Calculated MW 205kDa Observed MW 290kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location nuclear envelope, nuclear membrane, nuclear pore 
Western blot analysis of extracts from normal (control) and NUP210 knockout (KO) 293T cells, using NUP210 antibody (A19895) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 680-880 of human NUP210 (NP_079199.2). KO Validated Validated Isotype IgG