![[KO Validated] SCYL1 Rabbit pAb (A19913)](/upload/resize_cache/iblock/1f0/340_340_140cd750bba9870f18aada2478b24840a/q69l2gmzsgnbi4uuk5iajewq3nfeou4t.jpg "[KO Validated] SCYL1 Rabbit pAb (A19913)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SCYL1 Rabbit pAb (A19913)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SCYL1 Rabbit pAb Catalog No. A19913 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3). Sequence SASTRHRVLTSAFSRATRDPFAPSRVAGVLGFAATHNLYSMNDCAQKILPVLCGLTVDPEKSVRDQAFKAIRSFLSKLESVSEDPTQLEEVEKDVHAASSPGMGGAAASWAGWAVTGVSSLTSKLIRSHPTTAPTETNIPQRPTPEGVPAPAPTPVPATPTTSGHWETQEEDKDTAEDSSTADRWDDEDWGSLEQEAESVLAQQDDWSTGGQVSRASQVSNSDHKSSKSPESDWSSWEAEGSWEQGWQEPSSQEPPPDGTRLASEYNWGGPESSDKGDPFA Gene ID Swiss Prot Synonyms GKLP; NKTL; NTKL; P105; TAPK; TEIF; TRAP; HT019; SCAR21 Calculated MW 90kDa Observed MW 105kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Nucleus, centrosome, cis-Golgi network, cytoskeleton, microtubule organizing center 
Western blot analysis of extracts from normal (control) and SCYL1 knockout (KO) 293T cells, using SCYL1 antibody (A19913) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3). KO Validated Validated Isotype IgG