[KO Validated] CD146/MCAM Rabbit pAb (A19993)

быстрая доставка ~4 недели

Размер

100 μL
200 μL
50 μL

Описание
Характеристики

Overview

Product name	[KO Validated] CD146/MCAM Rabbit pAb
Catalog No.	A19993
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 340-560 of human CD146/CD146/MCAM (NP_006491.2).
Sequence	VNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRGV
Gene ID
Swiss Prot
Synonyms	CD146; MUC18; HEMCAM; METCAM; MelCAM
Calculated MW	72kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa
Cellular location	Membrane, Single-pass type I membrane protein

Western blot analysis of extracts from normal (control) and CD146/CD146/MCAM knockout (KO) HeLa cells, using CD146/CD146/MCAM antibody (A19993) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Key applications	Western blotting
Host species	Кролик
Antibody type	Primary Antibodies
Reactivity	Человек, Мышь
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 340-560 of human CD146/CD146/MCAM (NP_006491.2).
KO Validated	Validated
Isotype	IgG