![[KO Validated] YAP1 Rabbit mAb (A21216)](/upload/resize_cache/iblock/992/340_340_140cd750bba9870f18aada2478b24840a/lq5ltfa0l81spih755m22e1r6qq4rvmk.jpg "[KO Validated] YAP1 Rabbit mAb (A21216)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] YAP1 Rabbit mAb (A21216)
- Описание
- Характеристики
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Overview
Product name [KO Validated] YAP1 Rabbit mAb Catalog No. A21216 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53479 Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1). Sequence PTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL Gene ID Swiss Prot Synonyms YAP; YKI; COB1; YAP2; YAP-1; YAP65 Calculated MW 54kDa Observed MW 73kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP ChIP Recommended dilution - WB 1:2000 - 1:20000
- IHC-P 1:100 - 1:500
- IP 1:2000 - 1:10000
- IF/ICC 1:50 - 1:200
- ChIP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Immunofluorescence Positive samples HeLa, Mouse heart, C6 Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa(KO) cells, using YAP1 antibody (A21216) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of C6, using YAP1 antibody (A21216) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of Mouse heart, using YAP1 antibody (A21216) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human colon (low expression samples) using [KO Validated] YAP1 Rabbit mAb (A21216) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KO Validated] YAP1 Rabbit mAb (A21216) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of HeLa cells using [KO Validated] YAP1 Rabbit mAb (A21216, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg YAP1 antibody (A21216). Western blot was performed from the immunoprecipitate using YAP1 antibody (A21216) at a dilution of1:2000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using YAP1 antibody (A21216) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1). KO Validated Validated Isotype IgG