![[KO Validated] STRAP Rabbit pAb (A21351)](/upload/resize_cache/iblock/ff8/340_340_140cd750bba9870f18aada2478b24840a/enbk2k631rd3g7gbzpmn5uigi12mdbwe.jpg "[KO Validated] STRAP Rabbit pAb (A21351)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] STRAP Rabbit pAb (A21351)
- Описание
- Характеристики
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Overview
Product name [KO Validated] STRAP Rabbit pAb Catalog No. A21351 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables RNA binding activity. Involved in maintenance of gastrointestinal epithelium; negative regulation of transforming growth factor beta receptor signaling pathway; and spliceosomal snRNP assembly. Located in cytosol. Part of SMN complex. Implicated in adenocarcinoma; colorectal carcinoma; large cell carcinoma; lung carcinoma; and squamous cell neoplasm.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human STRAP (NP_009109.3). Sequence MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA Gene ID Swiss Prot Synonyms MAWD; PT-WD; UNRIP Calculated MW 38kDa Observed MW 39kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples 293T, HeLa Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from wild type(WT) and STRAP knockout (KO) HeLa cells, using STRAP Rabbit pAb (A21351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of H9C2 cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg STRAP antibody (A21351). Western blot was performed from the immunoprecipitate using STRAP antibody (A21351) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human STRAP (NP_009109.3). KO Validated Validated Isotype IgG