![[KO Validated] eIF4EBP1 Rabbit pAb (A21386)](/upload/resize_cache/iblock/9b6/340_340_140cd750bba9870f18aada2478b24840a/5gq8j2islzotj7qkagw8mlk3blqig4ix.jpg "[KO Validated] eIF4EBP1 Rabbit pAb (A21386)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] eIF4EBP1 Rabbit pAb (A21386)
- Описание
- Характеристики
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Overview
Product name [KO Validated] eIF4EBP1 Rabbit pAb Catalog No. A21386 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.Immunogen information
Immunogen Recombinant protein of human eIF4EBP1. Sequence Email for sequence Gene ID Swiss Prot Synonyms BP-1; 4EBP1; 4E-BP1; PHAS-I Calculated MW 13kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Cellular location cytoplasm, cytosol 
Western blot analysis of various lysates, using eIF4EBP1 antibody (A21386) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts from wild type(WT) and eIF4EBP1 knockout (KO) HeLe(KO) cells, using eIF4EBP1 antibody (A21386) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using eIF4EBP1 Rabbit pAb (A21386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human eIF4EBP1. KO Validated Validated Isotype IgG