![[KO Validated] STAT5B Rabbit pAb (A21613)](/upload/resize_cache/iblock/f7a/340_340_140cd750bba9870f18aada2478b24840a/hnqhfji3x5t0o552pw661rmzl4r0yo97.jpg "[KO Validated] STAT5B Rabbit pAb (A21613)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] STAT5B Rabbit pAb (A21613)
- Описание
- Характеристики
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Overview
Product name [KO Validated] STAT5B Rabbit pAb Catalog No. A21613 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 586-787 of human STAT5B (NP_036580.2). Sequence KPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS Gene ID Swiss Prot Synonyms STAT5; GHISID2 Calculated MW 90kDa Observed MW 92kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IP 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Immunofluorescence Positive samples HeLa, Mouse liver, Mouse heart, Mouse thymus, Rat liver Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using STAT5B antibody (A21613) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from wild type (WT) and STAT5B knockout (KO) HeLa cells, using STAT5B antibody (A21613) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse intestin using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat intestine using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of Mouse thymus cells using 3 μg STAT5B antibody (A21613). Western blot was performed from the immunoprecipitate using STAT5B antibody (A21613) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 586-787 of human STAT5B (NP_036580.2). KO Validated Validated Isotype IgG