![[KO Validated] SQSTM1/p62 Rabbit pAb (A21702)](/upload/resize_cache/iblock/20b/340_340_140cd750bba9870f18aada2478b24840a/mft4bvm5822057hr0y81t1qff0wa5kyj.jpg "[KO Validated] SQSTM1/p62 Rabbit pAb (A21702)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SQSTM1/p62 Rabbit pAb (A21702)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SQSTM1/p62 Rabbit pAb Catalog No. A21702 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.Immunogen information
Immunogen Recombinant protein of human SQSTM1/p62. Sequence Email for sequence Gene ID Swiss Prot Synonyms p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3 Calculated MW 48kDa Observed MW 62kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:2000 - 1:4000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples 293T, HeLa, L929, PC-12 Cellular location Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome 
Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A21702) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts from wild type(WT) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody (A21702) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using SQSTM1/p62 antibody (A21702) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Human extranodal NK-T cell lymphoma using SQSTM1/p62 antibody (A21702) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SQSTM1/p62 antibody (A21702) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using SQSTM1/p62 antibody (A21702) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 antibody (A21702) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using SQSTM1/p62 antibody (A21702) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-549 cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (A21702) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg SQSTM1/p62 antibody (A21702). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A21702) at a dilution of 1:1000.
Immunoprecipitation analysis of 600 μg extracts of Mouse liver using 3 μg SQSTM1/p62 antibody (A21702). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A21702) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human SQSTM1/p62. KO Validated Validated Isotype IgG