![[KO Validated] CD33 Rabbit pAb (A21714)](/upload/resize_cache/iblock/1b9/340_340_140cd750bba9870f18aada2478b24840a/l27b6i50cegwjh2w7ug4vbg9ryoyhigq.jpg "[KO Validated] CD33 Rabbit pAb (A21714)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CD33 Rabbit pAb (A21714)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CD33 Rabbit pAb Catalog No. A21714 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human CD33 (NP_001763.3). Sequence YYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH Gene ID Swiss Prot Synonyms p67; SIGLEC3; SIGLEC-3 Calculated MW 40kDa Observed MW 67kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cell membrane, Single-pass type I membrane protein 
Western blot analysis of extracts from wild type (WT) and CD33 knockout (KO) HeLa U14 CD33 antibody (A21714) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human CD33 (NP_001763.3). KO Validated Validated Isotype IgG