![[KO Validated] ZMYND8 Rabbit pAb (A21848)](/upload/resize_cache/iblock/359/340_340_140cd750bba9870f18aada2478b24840a/y93zg40kicvn4cj8f7nyh1dfa4x60xzh.jpg "[KO Validated] ZMYND8 Rabbit pAb (A21848)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] ZMYND8 Rabbit pAb (A21848)
- Описание
- Характеристики
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Overview
Product name [KO Validated] ZMYND8 Rabbit pAb Catalog No. A21848 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1). Sequence LELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD Gene ID Swiss Prot Synonyms RACK7; PRKCBP1; PRO2893 Calculated MW 132kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples Cellular location cytoplasm, Golgi apparatus, nucleoplasm, nucleus 
Western blot analysis of extracts from normal (control) and ZMYND8 knockout (KO) HeLa cells, using ZMYND8 antibody (A21848) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunoprecipitation analysis of 300 μg extracts of U-87 cells using 3 μg [KO Validated] ZMYND8 Rabbit pAb (A21848). Western blot was performed from the immunoprecipitate using [KO Validated] ZMYND8 Rabbit pAb (A21848) at a dilition of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1). KO Validated Validated Isotype IgG