![[KO Validated] ZEB1 Rabbit pAb (A22244)](/upload/resize_cache/iblock/586/340_340_140cd750bba9870f18aada2478b24840a/05q6vymmmcadeqybn41t4krkv72u20ei.jpg "[KO Validated] ZEB1 Rabbit pAb (A22244)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] ZEB1 Rabbit pAb (A22244)
- Описание
- Характеристики
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Overview
Product name [KO Validated] ZEB1 Rabbit pAb Catalog No. A22244 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.Immunogen information
Immunogen Recombinant protein of human ZEB1 Sequence Email for sequence Gene ID Swiss Prot Synonyms BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1 Calculated MW 124kDa Observed MW 200kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Jurkat, Rat heart Cellular location cytosol, nucleoplasm, nucleus 
Western blot analysis of extracts from normal (control) and ZEB1 knockout (KO) HeLa cells, using ZEB1 antibody (A22244) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of various lysates, using ZEB1 antibody (A22244) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant protein of human ZEB1 KO Validated Validated Isotype IgG