![[KO Validated] IGF2BP1/IMP1 Rabbit pAb (A22246)](/upload/resize_cache/iblock/9a7/340_340_140cd750bba9870f18aada2478b24840a/ujpks2l7be5i6kf4lyugu13vb4bj39gu.jpg "[KO Validated] IGF2BP1/IMP1 Rabbit pAb (A22246)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] IGF2BP1/IMP1 Rabbit pAb (A22246)
- Описание
- Характеристики
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Overview
Product name [KO Validated] IGF2BP1/IMP1 Rabbit pAb Catalog No. A22246 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human IMP1 Sequence Email for sequence Gene ID Swiss Prot Synonyms IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1 Calculated MW 63kDa Observed MW 64kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples HepG2, 293F, NIH/3T3, Mouse brain, Rat kidney Cellular location CRD-mediated mRNA stability complex, cytoplasm, cytoplasmic stress granule, cytosol, dendritic spine, growth cone, nucleoplasm, nucleus, P-body, perinuclear region of cytoplasm 
Western blot analysis of extracts from normal (control) and knockdown (KD) HepG2 cells, using ZEB1 antibody (A22246) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of 293F, using IMP1 antibody (A22246) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of various lysates, using IMP1 antibody (A22246) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human IMP1 KO Validated Validated Isotype IgG