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[KO Validated] CDKN1A/p21 Rabbit PolymAb® (A22460)

быстрая доставка ~4 недели

Размер

100 μL
200 μL
50 μL

Описание
Характеристики

Overview

Product name	[KO Validated] CDKN1A/p21 Rabbit PolymAb®
Catalog No.	A22460
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51040_ARC52072

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Immunogen information

Immunogen	Recombinant protein of human/mouse CDKN1A/p21.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Calculated MW	18kDa
Observed MW	21kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IF/ICC
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	RAW264.7, NIH/3T3, MCF7, 293T,
Cellular location	cytosol, nuclear body, nucleolus, nucleoplasm, nucleus

Western blot analysis of RAW264.7, using [KO Validated] CDKN1A/p21 Rabbit PolymAb® (A22460) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates, using [KO Validated] CDKN1A/p21 Rabbit PolymAb® (A22460) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of extracts from wild type(WT) and CDKN1A/p21 knockout (KO) 293T(KO) cells, using [KO Validated] CDKN1A/p21 Rabbit PolymAb® (A22460) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Confocal imaging of NIH/3T3 cells using [KO Validated] CDKN1A/p21 Rabbit PolymAb® (A22460, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Key applications	Western blotting, Immunofluorescence
Host species	Кролик
Antibody type	Primary Antibodies
Reactivity	Человек, Мышь
Immunogen	Recombinant protein of human/mouse CDKN1A/p21.
KO Validated	Validated
Isotype	IgG