![[KO Validated] Smad5 Rabbit mAb (A22749)](/upload/resize_cache/iblock/a0d/340_340_140cd750bba9870f18aada2478b24840a/amsajaoejydb3n642uwbl267unl5ullp.jpg "[KO Validated] Smad5 Rabbit mAb (A22749)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Smad5 Rabbit mAb (A22749)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Smad5 Rabbit mAb Catalog No. A22749 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC55344 Background
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant protein of human Smad5. Sequence Email for sequence Gene ID Swiss Prot Synonyms DWFC; JV5-1; MADH5 Calculated MW 52kDa Observed MW 60kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from wild type(WT) and Smad5 knockout (KO) 293T(KO) cells, using Smad5 antibody (A22749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant protein of human Smad5. KO Validated Validated Isotype IgG