![[KO Validated] α-Catenin Rabbit pAb (A5635)](/upload/resize_cache/iblock/72a/340_340_140cd750bba9870f18aada2478b24840a/uia3m84dmkq1oj77aqw82evwk7ftl3e7.jpg "[KO Validated] α-Catenin Rabbit pAb (A5635)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] α-Catenin Rabbit pAb (A5635)
- Описание
- Характеристики
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Overview
Product name [KO Validated] α-Catenin Rabbit pAb Catalog No. A5635 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human α-Catenin (NP_001894.2). Sequence ELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI Gene ID Swiss Prot Synonyms MDBS2; MDPT2; CAP102 Calculated MW 100kDa Observed MW 115kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples BT-474, OVCAR3, 293T, MCF7, Mouse lung, Mouse heart, Mouse brain Cellular location Cell junction, Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton Customer validation WB(Toxoplasma gondii, Mus musculus, Homo sapiens)
Western blot analysis of extracts of various cell lines, using α-Catenin antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts from normal (control) and α-Catenin Rabbit pAb knockout (KO) 293T cells, using α-Catenin Rabbit pAb antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human vermiform appendix using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg α-Catenin antibody (A5635). Western blot was performed from the immunoprecipitate using α-Catenin antibody (A5635) at a dilution of 1:500.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human α-Catenin (NP_001894.2). KO Validated Validated Isotype IgG