![[KO Validated] CDK5 Rabbit pAb (A5730)](/upload/resize_cache/iblock/bd8/340_340_140cd750bba9870f18aada2478b24840a/hz19l5y3wlvsn6dm12452f5yy029ld2u.jpg "[KO Validated] CDK5 Rabbit pAb (A5730)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CDK5 Rabbit pAb (A5730)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CDK5 Rabbit pAb Catalog No. A5730 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 170-289 of human CDK5 (NP_004926.1). Sequence PDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDF Gene ID Swiss Prot Synonyms LIS7; PSSALRE Calculated MW 33kDa Observed MW 30kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:10 - 1:100
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples 293T, HepG2, BT-474, A-549, PC-12, Mouse brain, Rat brain Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, Nucleus, Perikaryon, Peripheral membrane protein, growth cone, lamellipodium, postsynaptic cell membrane, postsynaptic density, synapse Customer validation WB(Mus musculus, Homo sapiens)
IP(Mus musculus)
Western blot analysis of extracts of various cell lines, using CDK5 antibody (A5730) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using CDK5 antibody (A5730) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and CDK5 knockout (KO) 293T cells, using CDK5 antibody (A5730) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using CDK5 antibody (A5730). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 900 μg extracts of A-549 cells using 3 μg CDK5 antibody (A5730). Western blot was performed from the immunoprecipitate using CDK5 antibody (A5730) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 170-289 of human CDK5 (NP_004926.1). KO Validated Validated Isotype IgG