![[KO Validated] CSDE1 Rabbit pAb (A5941)](/upload/resize_cache/iblock/d78/340_340_140cd750bba9870f18aada2478b24840a/ivdljd7bm50h8kxbrn5hy81y2t0it798.jpg "[KO Validated] CSDE1 Rabbit pAb (A5941)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CSDE1 Rabbit pAb (A5941)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CSDE1 Rabbit pAb Catalog No. A5941 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables RNA stem-loop binding activity. Involved in IRES-dependent viral translational initiation; nuclear-transcribed mRNA catabolic process, no-go decay; and stress granule assembly. Located in Golgi apparatus; cytosol; and plasma membrane. Part of CRD-mediated mRNA stability complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4). Sequence LSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEEGDMKGEV Gene ID Swiss Prot Synonyms UNR; D1S155E Calculated MW 89kDa Observed MW 89kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa Cellular location Cytoplasm Customer validation WB(Homo sapiens)
RIP(Homo sapiens)
Western blot analysis of extracts from normal (control) and CSDE1 knockout (KO) HeLa cells, using CSDE1 antibody (A5941) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
Immunofluorescence analysis of L929 cells using CSDE1 Rabbit pAb (A5941) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 μg extracts of HeLa cells using 3 μg CSDE1 antibody (A5941). Western blot was performed from the immunoprecipitate using CSDE1 antibody (A5941) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4). KO Validated Validated Isotype IgG