![[KO Validated] TAGLN2 Rabbit pAb (A6761)](/upload/resize_cache/iblock/ebc/340_340_140cd750bba9870f18aada2478b24840a/81q6fps5st8bvwdzxxyfj8wm5ktpbcrl.jpg "[KO Validated] TAGLN2 Rabbit pAb (A6761)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TAGLN2 Rabbit pAb (A6761)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TAGLN2 Rabbit pAb Catalog No. A6761 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1). Sequence MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL Gene ID Swiss Prot Synonyms HA1756 Calculated MW 22kDa Observed MW 22kDa Applications
Reactivity Human, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, A-549, Mouse heart Cellular location cytoskeleton, cytosol, extracellular exosome, extracellular region 
Western blot analysis of extracts from normal (control) and TAGLN2 knockout (KO) 293T cells, using TAGLN2 antibody (A6761) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of C6 cells using [KO Validated] TAGLN2 Rabbit pAb (A6761) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1). KO Validated Validated Isotype IgG