![[KO Validated] TLK2 Rabbit pAb (A6770)](/upload/resize_cache/iblock/aa8/340_340_140cd750bba9870f18aada2478b24840a/apn97u3oh7rogv23nwyvtdspzqqbgt1o.jpg "[KO Validated] TLK2 Rabbit pAb (A6770)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TLK2 Rabbit pAb (A6770)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TLK2 Rabbit pAb Catalog No. A6770 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2). Sequence MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISAL Gene ID Swiss Prot Synonyms HsHPK; MRD57; PKU-ALPHA Calculated MW 88kDa Observed MW 87kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, Mouse testis, NIH/3T3 Cellular location Cytoplasm, Nucleus, cytoskeleton, perinuclear region 
Western blot analysis of extracts of various cell lines, using TLK2 antibody (A6770) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and TLK2 Rabbit pAb knockout (KO) NIH/3T3 cells, using TLK2 Rabbit pAb antibody (A6770) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2). KO Validated Validated Isotype IgG