![[KO Validated] XIAP Rabbit pAb (A6869)](/upload/resize_cache/iblock/dac/340_340_140cd750bba9870f18aada2478b24840a/4e4lel7gv3k80ajstg1b4dfq2mn5bq5y.jpg "[KO Validated] XIAP Rabbit pAb (A6869)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] XIAP Rabbit pAb (A6869)
- Описание
- Характеристики
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Overview
Product name [KO Validated] XIAP Rabbit pAb Catalog No. A6869 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 198-497 of human XIAP (NP_001158.2). Sequence VQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS Gene ID Swiss Prot Synonyms API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3 Calculated MW 57kDa Observed MW 54kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples MCF-7, HeLa, SW620, HepG2, Jurkat, Mouse liver, Rat kidney Cellular location Cytoplasm, Nucleus Customer validation WB(Mus musculus, Homo sapiens)
IHC(Mus musculus)
IF(Mus musculus)
Western blot analysis of extracts of various cell lines, using XIAP antibody (A6869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (A6869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using XIAP antibody (A6869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 198-497 of human XIAP (NP_001158.2). KO Validated Validated Isotype IgG