![[KO Validated] SAMHD1 Rabbit pAb (A7794)](/upload/resize_cache/iblock/11a/340_340_140cd750bba9870f18aada2478b24840a/fkgiajiu15j6vy7orp0ygep5spexbgi6.jpg "[KO Validated] SAMHD1 Rabbit pAb (A7794)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SAMHD1 Rabbit pAb (A7794)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SAMHD1 Rabbit pAb Catalog No. A7794 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2). Sequence TDAFLKADDYIEITGAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEYRNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAEDFIVDVINMDYGMQEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAEQLIRVYCKKVDRKSLYAARQYFVQWCADRNFTKPQDGDVIAPLITPQKKEWNDSTSVQNPTRLREASKSRVQLFKDDPM Gene ID Swiss Prot Synonyms DCIP; CHBL2; HDDC1; MOP-5; SBBI88; hSAMHD1 Calculated MW 72kDa Observed MW 72kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples HepG2, HeLa, MCF-7, Mouse spleen Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (A7794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (A7794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using [KO Validated] SAMHD1 Rabbit pAb (A7794) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SAMHD1 antibody (A7794). Western blot was performed from the immunoprecipitate using SAMHD1 antibody (A7794) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2). KO Validated Validated Isotype IgG