![[KO Validated] UBE2O Rabbit pAb (A10036)](/upload/resize_cache/iblock/3dd/340_340_140cd750bba9870f18aada2478b24840a/haysp1da1wtucn4q2r3gxr8088i12d1v.jpg "[KO Validated] UBE2O Rabbit pAb (A10036)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] UBE2O Rabbit pAb (A10036)
- Описание
- Характеристики
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Overview
Product name [KO Validated] UBE2O Rabbit pAb Catalog No. A10036 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). Sequence LVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK Gene ID Swiss Prot Synonyms E2-230K Calculated MW 141kDa Observed MW 200kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:1000 - 1:4000
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples A-431, HepG2, Jurkat, Mouse heart, Mouse brain Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using UBE2O antibody (A10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (A10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using UBE2O Polyclonal Antibody (A10036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of A-431 cells using 3 μg UBE2O antibody (A10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (A10036) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). KO Validated Validated Isotype IgG