![[KO Validated] Hsp90β Rabbit pAb (A1087)](/upload/resize_cache/iblock/e30/340_340_140cd750bba9870f18aada2478b24840a/kvv3cn9obopfp65p94q3c32358h5sn9f.jpg "[KO Validated] Hsp90β Rabbit pAb (A1087)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Hsp90β Rabbit pAb (A1087)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Hsp90β Rabbit pAb Catalog No. A1087 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). Sequence MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE Gene ID Swiss Prot Synonyms HSP84; HSPC2; HSPCB; D6S182; HSP90B Calculated MW 83kDa Observed MW 80kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples K-562, DU145, SW620, COS-7, NIH/3T3, HeLa Cellular location Cytoplasm, Melanosome Customer validation IF(Human liver cell)
WB(Human liver cell, Rat cells, Rattus norvegicus, Gallus gallus, Mus musculus, Homo sapiens)
IHC(Mouse Embryonic Stem Cells)
Western blot analysis of extracts from normal (control) and Hsp90β knockout (KO) HeLa cells, using Hsp90β antibody (A1087) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). KO Validated Validated Isotype IgG