![[KO Validated] RALBP1 Rabbit pAb (A1140)](/upload/resize_cache/iblock/979/340_340_140cd750bba9870f18aada2478b24840a/jwlsxd362oh0qk1cnq3z88389w5dsxad.jpg "[KO Validated] RALBP1 Rabbit pAb (A1140)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] RALBP1 Rabbit pAb (A1140)
- Описание
- Характеристики
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Overview
Product name [KO Validated] RALBP1 Rabbit pAb Catalog No. A1140 Host species Rabbit Purification method Affinity purification Isotype IgG Background
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1). Sequence AGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAKEQPKAGKEPAKPSPSRDRKETSI Gene ID Swiss Prot Synonyms RIP1; RLIP1; RLIP76 Calculated MW 76kDa Observed MW 100kDa Applications
Reactivity Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-673, Mouse spleen, Mouse lung Cellular location Membrane, Peripheral membrane protein 
Western blot analysis of extracts of various cell lines, using RALBP1 antibody (A1140) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and RALBP1 knockout (KO) HeLa cells, using RALBP1 antibody (A1140) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1). KO Validated Validated Isotype IgG