![[KO Validated] EGFR Rabbit pAb (A11577)](/upload/resize_cache/iblock/3fa/340_340_140cd750bba9870f18aada2478b24840a/9jgx09xkisjdyu70sir9ior3c2wnoj2e.jpg "[KO Validated] EGFR Rabbit pAb (A11577)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] EGFR Rabbit pAb (A11577)
- Описание
- Характеристики
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Overview
Product name [KO Validated] EGFR Rabbit pAb Catalog No. A11577 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2). Sequence QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA Gene ID Swiss Prot Synonyms ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2 Calculated MW 134kDa Observed MW 175kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa Cellular location Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein Customer validation WB(Homo sapiens, Mus musculus, Rattus norvegicus)
IP(Homo sapiens)
Western blot analysis of extracts from wild type(WT) and EGFR Rabbit pAb knockout (KO) HeLa cells, using EGFR Rabbit pAb antibody (A11577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using [KO Validated] EGFR Rabbit pAb (A11577) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using [KO Validated] EGFR Rabbit pAb (A11577) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-431 cells using [KO Validated] EGFR Rabbit pAb (A11577) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2). KO Validated Validated Isotype IgG