![[KO Validated] Vimentin Rabbit pAb (A11952)](/upload/resize_cache/iblock/175/340_340_140cd750bba9870f18aada2478b24840a/eacw5m0no07xv1nlx4brlxytpbq1weyo.jpg "[KO Validated] Vimentin Rabbit pAb (A11952)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Vimentin Rabbit pAb (A11952)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Vimentin Rabbit pAb Catalog No. A11952 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 10-101 of human Vimentin (NP_003371.2). Sequence SYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT Gene ID Swiss Prot Synonyms CTRCT30; HEL113; Vimentin; VIM; vimentin Calculated MW 54kDa Observed MW 57kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples 293T, Jurkat, NIH/3T3, C6, Mouse testis Cellular location Cytoplasm Customer validation WB(Human glioma cells, Homo sapiens, Mus musculus, Canis lupus familiaris)
IF(Homo sapiens)
IHC(Homo sapiens, Mus musculus)
IF(Homo sapiens)
IHC(Homo sapiens)
Western blot analysis of extracts of various cell lines, using Vimentin antibody (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using [KO Validated] Vimentin Rabbit pAb (A11952) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 10-101 of human Vimentin (NP_003371.2). KO Validated Validated Isotype IgG