![[KO Validated] CD168/RHAMM Rabbit pAb (A12445)](/upload/resize_cache/iblock/8f5/340_340_140cd750bba9870f18aada2478b24840a/e8va3at6bgwbou8obnbt05u1ebs5avcw.jpg "[KO Validated] CD168/RHAMM Rabbit pAb (A12445)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CD168/RHAMM Rabbit pAb (A12445)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CD168/RHAMM Rabbit pAb Catalog No. A12445 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). Sequence MSFPKAPLKRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDKDTTLPASARKVKSSESKKESQKNDKDLKILEKEIRVLLQERGAQDRRIQDLETELEKMEARLNAALREKTSLSANNATLEKQLIELTRTNELLKSKFSENGNQKNLRILSLELMKLRNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQGKIAQLEGKLVSIEKEKIDEKSETEKLLEYIEEISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQSLEENIVILSKQVEDLNVKCQLLEKE Gene ID Swiss Prot Synonyms CD168; IHABP; RHAMM Calculated MW 84kDa Observed MW 84kDa Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples SW480, HeLa, Jurkat, ES-2 Cellular location Cell surface, Cytoplasm 
Western blot analysis of extracts of various cell lines, using CD168/RHAMM antibody (A12445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and CD168/RHAMM Rabbit pAb knockout (KO) HeLa cells, using CD168/RHAMM Rabbit pAb antibody (A12445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using CD168/RHAMM antibody (A12445). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). KO Validated Validated Isotype IgG